employee
Krasnoyarsk State Agrarian University (professor)
employee
Russian Federation
Russian Federation
VAK Russia 4.1.2
VAK Russia 4.1.3
VAK Russia 4.1.4
VAK Russia 4.1.5
VAK Russia 4.2.1
VAK Russia 4.2.2
VAK Russia 4.2.3
VAK Russia 4.2.4
VAK Russia 4.2.5
VAK Russia 4.3.3
VAK Russia 4.3.5
UDC 619
UDC 616.15
UDC 636.92
The aim of the study is to evaluate changes in chemiluminescent parameters of rabbit blood when ex-posed to subclinical doses of tritium. Objectives: to form subclinical absorbed doses in rabbits; to estimate the time to reach and the maximum intensity of the chemiluminescent reaction, the total production of pri-mary and secondary oxygen radicals in the peripheral blood of rabbits when exposed to tritium; to calculate the activation index of the chemiluminescent reaction. The work was carried out on Californian rabbits in the period 2021–2023 at the Institute of Applied Biotechnology and Veterinary Medicine of the Kras¬noyarsk State Agrarian University and the laboratory of the International Scientific Center for Research of Extreme States of the Body under the Presidium of the Federal Research Center of the Krasnoyarsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences. The absorbed doses formed for the study were: up to 2.6 mGy; 3.3–4.2; 6.4–14.0; 16.6–21.4; 50.9–52.2; 62.3–63.2; 92.6 and 355.6 mGy. The key parameters of ROS production were assessed: time to reach the maximum during the formation of the chemiluminescence curve peak; maximum intensity of the ROS generation peak; total ra-dical production; activation index. The dynamics of the total number of activated primary ROS were bi-modal, with a mode at doses of 3.3–4.2 and 62.3 mGy. When rabbits were exposed to tritium in the range from 2.6 to 62.3 mGy and at a dose of 355.6 mGy, the number of spontaneous primary oxygen radicals increased relative to intact groups. When exposed to tritium at a dose of 63 mGy, spontaneous and acti-vated production of primary ROS and spontaneous generation of secondary oxygen radicals increased, while a decrease in the IA of primary and secondary radicals was observed, which we define as a negative factor of tritium exposure. The observed changes confirm the theory of a bimodal relationship between parameters upon exposure to low doses of radiation.
peripheral blood, rabbits, oxygen free radicals, chemiluminescence, maximum reaction intensity
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