The regeneration peculiarities of four rice hybrids Oryza sativa L. subspecies japonica in the anther culture in vitro on the nutrient medium containing phenoxyacetic acid 10 mg/lare studied. The callus formation frequency on N 6 and М 8 mediums was equal (4,2% and 4,8%). In the two-step regeneration the albinoproportion after М 8 medium is two times higher than the after Nemedium (p - 0,01). In the one-step regeneration the green plants were not obtained. The possibility of inoculation of the aseptically clean rice anthers without sterilizing agents is shown.
in vitro, rice, phenoxyacetic acid, anther culture, callus, regeneration, in vitro
1. Niizeki B.H., Oono K. Induction of haploid rice plant from anther culture // Proc. Japan Acad. - 1968. -Vol. 44. - P. 554-557.
2. Chu C. The N6 medium and its applications to anther culture of cereal crops // Plant Tissue Culture. - 1978. - P. 43-50.
3. Datta S.K. Androgenic haploids: factors controlling development and its application in crop improvement // Current Science. - 2005. - Vol. 10. - P. 1870-1878.
4. Harchenko P.N., Kucherenko L.A. Poluchenie rasteniy risa iz pyl'nikov // Doklady VASHNIL. - 1977. -№ 4. - S. 15-16.
5. Zmeeva V.N., Zhuravlev Yu.N. Ispol'zovanie metodov biotehnologii v selekcii risa v Primorskom krae // Nauchnoe obespechenie APK Dal'nego Vostoka: mat-ly nauch. sessii (Ussuriysk, 18-20 avgusta 1993 g.). - Novosibirsk, 1995. - S. 132-136.
6. Goncharova Yu.K. Ispol'zovanie kul'tury pyl'nikov v selekcii risa. - Krasnodar, 2007. - 56 s.
7. Germana M.A. Gametic embryogenesis and haploid technology as valuable support to plant breeding // Plant Cell. Rep. - 2011. - Vol. 30. - P. 839-857.
8. Embriologicheskie osnovy androklinii pshenicy / N.N. Kruglova, T.B. Batygina, V.Yu. Gorbunova [i dr.]. - M.: Nauka, 2005. - 99 s.
9. Phenilacetic acid stimulation of direct shoot formation in anther and somatic tissue cultures of rice (Oryza sativa L.) / L.S. Zhuo, Si H.M, S.H. Cheng [et al.] // Plant Breeding. - 1996. - Vol. 115. - P. 295-300.
10. Analogues of phenoxyacetic acid and the generation of calluses from seeds of indica rice / T. Yasuda, S. Miyano, Y. Yamomoto [et al.] // Plant Cell Physiol. - 1990. - Vol. 31, № 6. - P. 763-766.
11. Abramova L.I. Opredelenie chisla hromosom i opisanie ih morfologii v meristeme i pyl'cevyh zernah kul'turnyh rasteniy: metod. ukazaniya. - L.: Izd-vo VNII rastenievodstva im. N.I. Vavilova (VIR), 1986. - 63 s.
12. Lakin G.F. Biometriya. - M.: Vyssh. shk., 1980. - 293 s.
13. Gorbunova V.Yu. Androgenez in vitro u yarovoy myagkoy pshenicy: avtoref. dis.. d-ra biol. nauk. - SPb., 2000. - 48 s.
14. Ferrie A.M., Caswell K.L. Isolated microsporeculture techniques and recent progress for haploid and doubled haploid plant production // Plant Cell Tiss. Organ Cult. - 2011. - Vol. 104. - P. 301-309.
15. Goncharova Yu.K. Nasledovanie priznaka «otzyvchivost' na kul'turu pyl'nikov» u risa // Vestnik Ros. akad. s.-h. nauk. - 2008. - № 2. - S. 40-42.
16. Teoreticheskie aspekty polucheniya gaploidov v kul'ture izolirovannyh pyl'nikov zlakov / T.I. D'yachuk, S.V. Tuchin, S.V. Stolyarova [i dr.] // Vestnik Ros. akad. s.-h. nauk. - 2007. - № 2. - S. 11-13.
17. Kruglova N.N. Innovacionnaya biotehnologiya androklinnoy gaploidii yarovoy myagkoy pshenicy: embriologicheskiy podhod // Agrarnaya Rossiya. - 2009. - № 1. - S. 34-38.