The luminescence and lipid peroxidation (LPO) intensities were studied in the samples of the mycelium of luminous fungus Neonothopanus nambi after mechanical damage and incubation under various conditions. By using nitroblue tetrazolium the activation of reactive oxygen species (ROS) in damaged mycelium samples was demonstrated by incubation in a nutrient medium as well as in deionized water. In both cases there was a significant (3-4 orders of magnitude) increase in the intensity of light emission of mycelium. Moreover, a higher level of emission was registered in mycelium samples incubated in deionized water. Based on the data about formation of colored complex with 2-thiobarbituric acid it was found that incubation of the mycelium in culture medium was accompanied by a significant (5-fold) accumulation of malondialdehyde (MDA) in fungal biomass compared to its initial level that favors the development of LPO. During the incubation of the mycelium in deionized water, the MDA changes in the biomass did not reveal. Since under incubation in the water the light emission intensity of mycelium was substantially higher (2-2,5-fold) than that in the mycelium in culture medium, the assumption was made that in this case the main excess of ROS, formed by fungus was neutralized during the luminescent reaction, and it prevented the development of LPO or reduced the probability of this process.
reactive oxygen species, lipid peroxidation, luminescence of fungi
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