The research objective was studying the main toxic ef-fects of new preparation "Vetom 21.77" on the basis of nema-tophage mushroom of Duddingtonia flagrans, struck target organs, latent period of the development and reversibility of effects. 5 groups (control and 4 experimental) having 20 mice in each were created. The experiment was made on the basis of Research Veterinary Laboratory of Agroscience and Tech-nology Park at the State University of Shakarim (Families, Kazakhstan). To experimental groups "Vetom 21.77" was appointed orally in the doses 2, 5, 50 and 300 mkl/kg masses daily for 6 months; in control group the preparation was not applied. For the results accounting of performed daily clinical examination, weekly weighing, registered the gain of absolute weight; studied the influence of the preparation on hematolog-ic indicators and carried out the morphometry of separate internal to research and through 1,5; 3; 4 and 6 months after application of the preparation. By the results of the research it was found out that at long using in the doses of 2, 5, 50 and 300 mkl/kg weight "Vetom 21.77" had no toxic impact on the organism of mice, i.e. absolute safety of animals was detect-ed. In the animals of experimental groups high gain of live weight was observed. According to the physiological state, hematological indices and morphometric parameters of stud-ied organs, there were no deviations from the norm in the treatment groups, i.e. Vetom 21.77 was defined as the fourth toxicity class drug - 'low-hazard substances'.
Duddingtonia flagrans, preparation "Vetom 21.77", chronic toxicity, dose, mice, nematophage mushroom Duddingtonia flagrans
1. Isolation and Characterization of China Isolates of Duddingtonia flagrans, a Candidate of the Nematophagous Fungi for Biocontrol of Animal Parasitic Nematodes / B. Wang, W. Liu, M. Chen M. [et al.] // J. Parasitol. - 2015. - Vol. 101, № 4. - P. 476-484.
2. Resistance of different fungal structures of Duddingtonia flagrans to the digestive process and predatory ability on larvae of Haemonchus contortus and Strongyloides papillosus in goat feces / A. Campos, J. Araújo, M. Guimarães [et al.] // Parasitol. Res. - 2009. - Vol. 105, № 4. - P. 913-919.
3. Efremova E.A., Teplyakova T.V. Vliyanie glubinnoy kul'tury hischnogo griba Duddingtonia flagrans na chislennost' lichinok strongilyat pischevaritel'nogo trakta ovec // Rossiyskiy parazitologicheskiy zhurnal. - 2010. - № 3. - S. 33-38.
4. Feeding horses with industrially manufactured pellets with fungal spores to promote nematode integrated control / J. Hernández, F. Arroyo, J. Suárez [et al.] // Vet. parasitol. - 2016. - Vol. 229. - P. 37-44.
5. Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials / C. Paraud, H. Hoste, Y. Lefrileux [et al.] // Vet. Res. - 2005. - Vol. 36, № 2. - P. 157-166.
6. GOST 32519-2013. Metody ispytaniy po vozdeystviyu himicheskoy produkcii na organizm cheloveka. Izuchenie hronicheskoy toksichnosti pri vnutrizheludochnom postuplenii. - M., 2014. - 16 s.
