The aim of the work was to isolate endophytic bacte-ria from the wood of the laurel poplar (Populus laurifolia Ldb.) and fragrant poplar (Populus suaveolens Fisch. The objectives of the study were to identify endophytic bacteria from the wood of the laurel poplar; isolate en-dophytic bacteria from the wood of the fragrant poplar; to identify microorganisms. Wood samples were taken in early October with sterile increment borer. The wood sawdust from the central part of the trunk was investi-gated by the method of growth. Meat and peptone agar was used as a nutrient medium. The sawdust of the laurel poplar is overgrown with bacteria more intensive- ly, in comparison with the poplar fragrant. To determine enzymatic (biochemical) activity of studied bacteria, differential-diagnostic media of Guiss were used. For isolates of bacteria the identification was carried out by sequencing fragments of the 16S рRNA gene. Bacterial DNA was isolated from the pure cultures of the bacterial strains. 16S рRNA gene fragment was amplified using primers F27 / 1492R. Identification of the bacteria was carried out by sequencing corresponding fragments of the 16S рRNA gene and comparing the resulting nucle-otide sequences with homologous ones from the NCBI database using BLAST programs. Four species strains of Bacillus pumilus, Lysinibacillus fusiformis, Staphylo-coccus pasteuri and Brenneria salicis have been isolat-ed from laurel poplar wood. Three species strains of Bacillus safensis, Brenneria populi were isolated from fragrant poplar wood. The strains of the species Bacillus pumilus, Bacillus safensis, Lysinibacillus fusiformis, Staphylococcus pasteuri are potentially economically valuable species of bacteria. Biochemical activity of isolated strains with the respect to maltose, glucose, sucrose, sorbitol, mannitol, and lactose was studied.
bacterial endophytes, laurel poplar, fra-grant poplar, wood
1. Bakulin V.T. Osobennosti anatomicheskogo stroeniya drevesiny topolya belogo, proizrastayuschego v Zapadnoy Sibiri // Nauchnye vedomosti. Ser. «Estestvennye nauki». - 2012. - T. 18, № 3 (122). - S. 5-14.
2. Ivannikov S.P. Topol'. - M.: Lesn. prom-st', 1980. - 82 s.
3. Cavkelova E.A., Cherdynceva T.A., Netrusov A.I. Obrazovanie auksinov bakteriyami, associirovannymi s kornyami orhidey // Mikrobiologiya. - 2005. - T. 74, № 1. - S. 55-62.
4. Maksimov I.V., Abizgil'dina R.R., Pusenkova L.I. Stimuliruyuschie rost rasteniy mikroorganizmy kak al'ternativa himicheskim sredstvam zaschity ot patogenov (obzor) // Prikladnaya biohimiya i mikrobiologiya. - 2011. - T. 47, № 4. - S. 373-385.
5. Taghavi S., Barac T., Greenberg B., Borremans B., Vangronsveld J., van der Lelie D. Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene // Appl. Environ. Microbiol. - 2005. - № 71. - R. 8500-8505.
6. Lelie D. van der, Taghavi S., Monchy S., Schwender J., Miller L., Ferrieri R., Rogers A., Wu X., Zhu W., Weyens N., Vangronsveld J., Newman L. Poplar and its Bacterial Endophytes: Coexist-ence and Harmony // Plant Science. - 2009. - № 28. - R. 346-358.
7. Melent'ev A.I. Aerobnye sporoobrazuyuschie bakterii Bacillus Cohn. v agroekosistemah. - M.: Nauka, 2007. - 147 s.
8. Margesin R., Schinner F. Potential of halotolerant and halophilic microorganisms for biotechnology // Extremophiles. - 2001. - Vol. 5. - P. 73-83.
9. Zhuang W.Q., Tay J.-H., Maszenan A.M., Tay S.T.L. Bacillus naphthalinovorans sp. nov.from oil-contaminated tropical marine sediments and its role in napththalene biodegradation // Appl. Micro-biol. Biotechnol. - 2002. - Vol. 58. - P. 547-553.
10. Ren J.-H., Li H., Wang Y.-F., Wu X.-Q. Biocontrol potential of an endophytic Bacillus pumilus JK-SX001 against poplar canker // Biological Control - 2013. - № 67 (3). - P. 421-430.
11. Cawoy H., Bettiol W., Fickers P., Ongena M. Bacil-lus - Based Biological Control of Plant Diseases // Pesticides in the Modern World - Pesticides Use and Management. - Rijeka: INTECH, 2011. - P. 273-302.
12. Mardanova A.M., Lutfullin M.T., Shalavina M.A. i dr. Poisk i vydelenie novyh shtammov bakteriy-antagonistov fitopatogennyh mikromicetov roda Fusarium // Bioraznoobrazie i ekologiya gribov i gribopodobnyh organizmov severnoy Evrazii: mat-ly vseros. konf. s mezhdunar. uchastiem. - Ekaterinburg: Izd-vo Ural. gos. un-ta, 2015. - S. 149-150.
13. Mayer F., Kronstad J.W. Breaking the bad: Bacillus blocks fungal virulence factors // Microbial Cell. - 2017. - № 4 (11). - P. 384-386.
14. Kothari V.V., Kothari R.K., Kothari C.R., Bhatt V.D.,Nathani N.M., Koringa P.G., Joshi C.G., Vyas B.R.M. Genome sequence of salt-tolerant bacillus safensis strain vk, isolated from saline desert area of Gujarat, India // Genome Announcements. - 2013. - № 1 (5). - P.e00671- e00683.
15. Kakade P.D., Chaphalkar S.R. Isolation and purifi-cation of antibacterial peptide from Bacillus safen-sis endophytica bacteria from Anthocephalus ka-damba // International Journal of Current Microbi-ology and Applied Sciences. - 2017. - Vol. 6, № 1. - P. 504-511.
16. Moore F.P., Barac T., Borremans B., Oeyen L., Vangronsveld J., van der Lelie D., Campbell C.D., Moore E.R.B. Endophytic bacterial diversity in pop-lar trees growing on a BTEX-contaminated site: the characterization of isolates with potential to en-hance phytoremediation // Syst Appl Microbiol. - 2012. - № 29. - P. 539-594.
17. Suhandono S., Kusumawardhani M.K., Aditiawati P. Isolation and molecular identification of endophytic bacteria from rambutan fruits (Nephelium lappaceum L.) cultivar binjai // HAYATI Journal of Biosciences. - 2016. - № 23. - P. 39-44.
18. Mehta J., Dilbaghi N., Dudeja S.S., Yadav A., Sharma P. Decolourization of simulated dye in aqueous medium using bacterial strains // Europe-an Journal of Advances in Engineering and Tech-nology. - 2015. - № 2 (3). - P. 9-18.
19. Cherpakov V.V. Bolezn' vodyanyh znakov («Wa-termark») na ivah (Salixspp.) v Rossii // Aktual'nye problemy lesnogo kompleksa. - 2015. - № 43. - S. 111-118.
20. Stefansson J. Screening for the occurrence of Brenneriasalicis in willows in Skåne, Sweden. - Alnarp: Swedish University or Agricultural Scienc-es, 2013. - 14 p.
