The Triton X-100detergent in fluence on the cholinesterase extractive capacity from the frozen and dehydrated squid ganglia is researched. The molecular mass of cholinesterase enzyme preparation is determined.
Triton X-100, cholinesterase, substrate specificity
1. Skoups R. Metody ochistki belkov. - M.: Mir, 1985. - 358 s.
2. Brestkin A.P., Vyazemskaya M.M., Mayzel' E.B. Vliyanie tritona H-100 na svoystva acetilholinesterazy iz eritrocitov krovi cheloveka // Biohimiya. - 1978. - T. 43, № 1. - S. 94-99.
3. Vydelenie i kataliticheskie svoystva rastvorimoy i membrannoy holinesterazy mozga kapustnoy muhi Delia brassicae / G.M. Grigor'eva, T.I. Krasnova, A.E. Hovanskih [i dr.] // Biohimiya. - 1987. -T. 52, № 7. - S. 1192-1200.
4. Rosenfeld C., Kousba A., Sultatos L.G. Interactions of rat brain acetylcholinesterase with the detergent Triton X-100 and the organophosphate paraoxon // Toxicological Sciences. - 2001. - Vol. 63, Iss 2. - P. 208-213.
5. Molecular properties of acetylcholinesterase in mouse spleen / S. Nietoceron, M.T. Moralnaranjo, E. Munozdelgado [et al.] // Neurochemistry International. - 2004. - Vol. 45, Iss 1. - P. 129-139.
6. Hussein A.S., Grigg M.E., Selkirk M.E. Nippostrongylus brasiliensis: Characterisation of a somatic am-phiphilic acetylcholinesterase with properties distinct from the secreted enzymes // Experimental Parasitology. - 1999. - Vol. 91, Iss 2. - P. 144-150.
7. Purification and characterization of acetylcholinesterase from oriental fruit fly (Bactrocera dorsalis (Hendel)) (Diptera: Tephritidae) / Y.M. Hsiao, J.Y. Lai, H.Y. Liao [et al.] // J. of Agricultural and Food Chemistry. - 2004. - Vol. 52, Iss 17. - P. 5340-5346
8. Amphiphilic and hydrophilic forms of acetylcholinesterase from sheep platelets / M.R. Marcos, J. Sanchez Yague, A. Hernandez Hernandez [et al.] // Biochimica et Biophysica Acta - Biomembranes. - 1998. - Vol. 1415, Iss 1. - P. 163-173.
9. A new and rapid colorimetric determination of acetylcholinesterase activity / G.L. Ellman, K.D. Courtney, V.Jr. Andres [et al.] // Biochem. Pharmacol. - 1961. - Vol. 7, № 1. - R. 88-95.
10. Kornish-Bouden E. Osnovy fermentativnoy kinetiki. - M.: Mir, 1979. - 280 s.
11. Kabachnik M.I. Fosfororganicheskie fiziologicheski aktivnye veschestva // Vestn. AN SSSR. - 1964. - № 40. - S. 60-68.
12. Yakovlev V.A. Kinetika fermentativnogo kataliza. - M : Nauka, 1965. - 248 s.
13. Osterman L.A. Metody issledovaniya belkov i nukleinovyh kislot. Elektroforez i ul'tracentrifugirovanie. - M.: Nauka, 1981. - 286 s.
14. Karnovsky M., Roots L.A. "Direct-colouring" thiocholine method for cholinesterases // Histochem. Cytochem. - 1964. - Vol. 12. - P. 219-226
15. Miheev E.V., Kovalev N.N. Tehnologicheskie harakteristiki gangliev kal'marov kak syr'ya dlya polucheniya fermenta holinesterazy // Izv. TINRO. - 2012. - T. 169. - S. 238-245.
