Yakutsk, Russian Federation
The research objective was studying of the possibil-ity of using rye flour in nutrient medium for receiving bacterial concentrate of microbic consortium. The re-search problems were 1) to study the influence of a dose (1, 2, 3 %) of rye flour on the growth of biomass and preservation in it balanced ratio of the main groups of microorganisms of microbic consortium; 2) to estab-lish influence of rye flour in a nutrient medium on aggre-gation and adhesion of microorganisms of microbic con-sortium; 3) to investigate qualitative characteristics of a bacterial concentrate of microbic consortium. As object of researches inoculated nutrient mediums on cottage cheese serum with different doses (1, 2, 3 %) of rye flour were used. As control nutrient medium without addition of rye flour served. The dynamics of growth of biomass of microbic consortium determined by optical density by photocolourmetric method on KF-77 λ = 550 n. Quantitative accounting of yeast and lactobacilli was carried out by the method of limiting dilution culture of the number of colony forming units (CFU/cm3) under on selective media by the method V.I. Brilis. As a result of researches it was established that introduction of 2 % of rye flour provided the greatest increase in optical densi-ty of the environment. Quantitative accounting of micro-organisms showed that in nutrient medium from 2 % of rye flour the greatest concentration of mesophilic lacto-bacilli to 5∙1011, CFU/cm3 and yeast, non-fermenting lactose, 3·108, CFU /cm3 was observed. The conditions of receiving bacterial concentrate provide the aggrega-tion of cells and increase of adhesive properties of microbic consortium (adhesiveness index 5.4+1). Study-ing of qualitative characteristic of bacterial concentrate testifies to its high biochemical activity.
lactobacilli, yeast, adhesion, bacterial concentrate, rye flour
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